Adsorption of human carbonic anhydrase II onto silicon oxides surfaces: The effects of truncation in the N-terminal region

Research output: Chapter in Book/Report/Conference proceedingPaper in conference proceeding

Abstract

The adsorption of human carbonic anhydrase II pseudo-wild type (HCAIIpwt) and an N-terminally truncated version thereof onto silica surfaces were studied. The amount adsorbed and the adsorption kinetics were measured using in situ ellipsometry. A substantial difference was seen between the two proteins. The adsorbed amount of the truncated version (2.53 mg/m2) indicates an end-on orientation, while the HCAIIpwt seems to adsorb side-on (1.84 mg/m2). It is suggested that the orientation effects arise from the truncation. The truncation is known to unfold the two most N-terminal helical segments, which could inhibit adsorption with the N-terminal region facing the surface, due to steric repulsion.

Details

Authors
Organisations
External organisations
  • University of Gothenburg
  • Linköping University
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Food Engineering

Keywords

Original languageEnglish
Title of host publicationThe Colloid Science of Lipids New Paradigms for Self-Assembly in Science and Technology
EditorsB. Lindman, B. W. Ninham
PublisherSpringer
Pages161-165
Number of pages5
Volume108
ISBN (Electronic)978-3-7985-1655-7
ISBN (Print)978-3-7985-1112-5
Publication statusPublished - 1998
Publication categoryResearch
Peer-reviewedYes

Publication series

NameProgress in Colloid & Polymer Science book series, PROGCOLLOID
PublisherSpringer
Volume108
ISSN (Print)0340-255X
ISSN (Electronic)1435-1536