Altered chloride homeostasis decreases the action potential threshold and increases hyperexcitability in hippocampal neurons

Research output: Contribution to journalArticle


Chloride ions play an important role in controlling excitability of principal neurons in the central nervous system. When neurotransmitter GABA is released from inhibitory interneurons, activated GABA type A (GABAA) receptors on principal neurons become permeable to chloride. Typically, chloride flows through activated GABAA receptors into the neurons causing hyperpolarization or shunting inhibition, and in turn inhibits action potential (AP) generation. However, in situations when intracellular chloride concentration is increased, chloride ions can flow in opposite direction, depolarize neurons, and promote AP generation. It is generally recognized that altered chloride homeostasis per se has no effect on the AP threshold. Here, we demonstrate that chloride overload of mouse principal CA3 pyramidal neurons not only makes these cells more excitable through GABAA receptor activation but also lowers the AP threshold, further aggravating excitability. This phenomenon has not been described in principal neurons and adds to our understanding of mechanisms regulating neuronal and network excitability, particularly in developing brain and during pathological situations with altered chloride homeostasis. This finding further broadens the spectrum of neuronal plasticity regulated by ionic compositions across the cellular membrane.


External organisations
  • Skåne University Hospital
  • University of Copenhagen
  • University of Cagliari
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Neurosciences


  • Action potential threshold, Chloride, ENpHR3.0, GABA receptors, Halorhodopsin, Optogenetics
Original languageEnglish
Article numbere0172-17.2017
Issue number6
Publication statusPublished - 2017 Nov 1
Publication categoryResearch