Altered processing of human insulin by B lymphocytes from an immunologically insulinresistant type I diabetic patient

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Bibtex

@article{229a267fd2ee4f94bde2125205cb701e,
title = "Altered processing of human insulin by B lymphocytes from an immunologically insulinresistant type I diabetic patient",
abstract = "Immunologically insulin resistant (IIR) type I diabetic patients possess significantly elevated levels of anti-insulin serum autoantibodies. We investigated whether altered insulin processing by B lymphocytes contributes to this form of insulin resistance. A comparison was made of the 125I-labelled human insulin (HI) peptides processed by Epstein-Barr virus (EBV)-transformed B lymphocytes derived from HLA-identical and nonidentical IIR with non-IIR type I diabetic patients on insulin therapy and healthy non-diabetic individuals. Several peptides detected in the extracellular, membrane-associated and intracellular compartments of B cells from an IIR type I diabetic patient differed from those found in the corresponding compartments of B cells from two non-IIR type I diabetic patients and two normal individuals. These data suggest that HI is processed differently by B cells from an IIR type I diabetic patient compared with B cells from non-IIR type I diabetic patients and normal individuals. Further, we found that two of the five plasma-membrane associated processed HI peptides on the IIR patients' B cells were absent from the membrane compartments of the other B cell lines examined. Thus, it is possible that one or both of these peptides, unique to the IIR patients' B cells, consist of an immundominant epitope(s) that stimulates the production of insulin autoantibodies which mediate the onset of IIR in type I diabetes.",
author = "Semple, {John W.} and Delovitch, {Terry L.}",
year = "1991",
month = "1",
day = "1",
doi = "10.1016/0896-8411(91)90024-7",
language = "English",
volume = "4",
pages = "277--289",
journal = "Journal of Autoimmunity",
issn = "0896-8411",
publisher = "Elsevier",
number = "2",

}