Amino acid supplementation improves heterologous protein production by Saccharomyces cerevisiae in defined medium

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Amino acid supplementation improves heterologous protein production by Saccharomyces cerevisiae in defined medium. / Görgens, Johann F; van Zyl, WH; Knoetze, JH; Hahn-Hägerdal, Bärbel.

In: Applied Microbiology and Biotechnology, Vol. 67, No. 5, 2005, p. 684-691.

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TY - JOUR

T1 - Amino acid supplementation improves heterologous protein production by Saccharomyces cerevisiae in defined medium

AU - Görgens, Johann F

AU - van Zyl, WH

AU - Knoetze, JH

AU - Hahn-Hägerdal, Bärbel

PY - 2005

Y1 - 2005

N2 - Supplementation of a chemically defined medium with amino acids or succinate to improve heterologous xylanase production by a prototrophic Saccharomyces cerevisiae transformant was investigated. The corresponding xylanase production during growth on ethanol in batch culture and in glucose-limited chemostat culture were quantified, as the native ADH2 promoter regulating xylanase expression was derepressed under these conditions. The addition of a balanced mixture of the preferred amino acids, Ala, Arg, Asn, Glu, Gln and Gly, improved both biomass and xylanase production, whereas several other individual amino acids inhibited biomass and/or xylanase production. Heterologous protein production by the recombinant yeast was also improved by supplementing the medium with succinate. The production of heterologous xylanase during growth on ethanol or glucose could thus be improved by supplementing metabolic precursors in the carbon- or nitrogen-metabolism.

AB - Supplementation of a chemically defined medium with amino acids or succinate to improve heterologous xylanase production by a prototrophic Saccharomyces cerevisiae transformant was investigated. The corresponding xylanase production during growth on ethanol in batch culture and in glucose-limited chemostat culture were quantified, as the native ADH2 promoter regulating xylanase expression was derepressed under these conditions. The addition of a balanced mixture of the preferred amino acids, Ala, Arg, Asn, Glu, Gln and Gly, improved both biomass and xylanase production, whereas several other individual amino acids inhibited biomass and/or xylanase production. Heterologous protein production by the recombinant yeast was also improved by supplementing the medium with succinate. The production of heterologous xylanase during growth on ethanol or glucose could thus be improved by supplementing metabolic precursors in the carbon- or nitrogen-metabolism.

U2 - 10.1007/s00253-004-1803-3

DO - 10.1007/s00253-004-1803-3

M3 - Article

C2 - 15630584

VL - 67

SP - 684

EP - 691

JO - Applied Microbiology and Biotechnology

JF - Applied Microbiology and Biotechnology

SN - 1432-0614

IS - 5

ER -