Amplification and overexpression of p40 subunit of eukaryotic translation initiation factor 3 in breast and prostate cancer

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Amplification and overexpression of p40 subunit of eukaryotic translation initiation factor 3 in breast and prostate cancer. / Nupponen, N N; Porkka, K; Kakkola, L; Tanner, Minna; Persson, Karin; Borg, Åke; Isola, Jorma; Visakorpi, T.

In: American Journal of Pathology, Vol. 154, No. 6, 1999, p. 1777-1783.

Research output: Contribution to journalArticle

Harvard

Nupponen, NN, Porkka, K, Kakkola, L, Tanner, M, Persson, K, Borg, Å, Isola, J & Visakorpi, T 1999, 'Amplification and overexpression of p40 subunit of eukaryotic translation initiation factor 3 in breast and prostate cancer', American Journal of Pathology, vol. 154, no. 6, pp. 1777-1783.

APA

Nupponen, N. N., Porkka, K., Kakkola, L., Tanner, M., Persson, K., Borg, Å., ... Visakorpi, T. (1999). Amplification and overexpression of p40 subunit of eukaryotic translation initiation factor 3 in breast and prostate cancer. American Journal of Pathology, 154(6), 1777-1783.

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MLA

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Author

Nupponen, N N ; Porkka, K ; Kakkola, L ; Tanner, Minna ; Persson, Karin ; Borg, Åke ; Isola, Jorma ; Visakorpi, T. / Amplification and overexpression of p40 subunit of eukaryotic translation initiation factor 3 in breast and prostate cancer. In: American Journal of Pathology. 1999 ; Vol. 154, No. 6. pp. 1777-1783.

RIS

TY - JOUR

T1 - Amplification and overexpression of p40 subunit of eukaryotic translation initiation factor 3 in breast and prostate cancer

AU - Nupponen, N N

AU - Porkka, K

AU - Kakkola, L

AU - Tanner, Minna

AU - Persson, Karin

AU - Borg, Åke

AU - Isola, Jorma

AU - Visakorpi, T

PY - 1999

Y1 - 1999

N2 - Amplification at the long arm of chromosome 8 occurs in a large fraction of breast and prostate cancers. To clone the target genes for this amplification, we used suppression subtraction hybridization to identify overexpressed genes in the breast cancer cell line SK-Br-3, which harbors amplification at 8q (8q21 and 8q23-q24). A differentially expressed gene identified by SSH, the p40 subunit of eukaryotic translation initiation factor 3 (eIF3), was localized to 8q23 and found to be highly amplified and overexpressed in the breast and prostate cancer cell lines studied. High-level amplification of eIF3-p40 was found in 30% of hormone-refractory prostate tumors and in 18% of untreated primary breast tumors. In the vast majority of the cases, p40 and c-myc were amplified with equal copy numbers. Tumors with higher copy numbers of p40 than c-myc were also found. Expression of p40 mRNA was analyzed with in situ hybridization. The amplification of eIF3-p40 gene was associated with overexpression of its mRNA, as expected for a functional target gene of the amplification. These results imply that genomic aberrations of translation initiation factors, such as eIF3-p40, may contribute to the pathogenesis of breast and prostate cancer.

AB - Amplification at the long arm of chromosome 8 occurs in a large fraction of breast and prostate cancers. To clone the target genes for this amplification, we used suppression subtraction hybridization to identify overexpressed genes in the breast cancer cell line SK-Br-3, which harbors amplification at 8q (8q21 and 8q23-q24). A differentially expressed gene identified by SSH, the p40 subunit of eukaryotic translation initiation factor 3 (eIF3), was localized to 8q23 and found to be highly amplified and overexpressed in the breast and prostate cancer cell lines studied. High-level amplification of eIF3-p40 was found in 30% of hormone-refractory prostate tumors and in 18% of untreated primary breast tumors. In the vast majority of the cases, p40 and c-myc were amplified with equal copy numbers. Tumors with higher copy numbers of p40 than c-myc were also found. Expression of p40 mRNA was analyzed with in situ hybridization. The amplification of eIF3-p40 gene was associated with overexpression of its mRNA, as expected for a functional target gene of the amplification. These results imply that genomic aberrations of translation initiation factors, such as eIF3-p40, may contribute to the pathogenesis of breast and prostate cancer.

M3 - Article

VL - 154

SP - 1777

EP - 1783

JO - American Journal of Pathology

T2 - American Journal of Pathology

JF - American Journal of Pathology

SN - 1525-2191

IS - 6

ER -