Amplification of 2p as a genomic marker for transformation in lymphoma

Research output: Contribution to journalArticle

Abstract

To outline further genetic mechanisms of transformation from follicular lymphoma (FL) to diffuse large B-cell lymphoma (DLBCL), we have performed whole genome array-CGH in 81 tumors from 60 patients [29 de novo DLBCL (dnDLBCL), 31 transformed DLBCL (tDLBCL), and 21 antecedent FL]. In 15 patients, paired tumor samples (primary FL and a subsequent tDLBCL) were available, among which three possessed more than two subsequent tumors, allowing us to follow specific genetic alterations acquired before, during, and after the transformation. Gain of 2p15-16.1 encompassing, among others, the REL, BCL11A, USP34, COMMD1, and OTX1 genes was found to be more common in the tDLBCL compared with dnDLBCL (P<0.001). Furthermore, a high-level amplification of 2p15-16.1 was also detected in the FL stage prior to transformation, indicating its importance during the transformation event. Quantitative real-time PCR showed a higher level of amplification of REL, USP34, and COMMD1 (all involved in the NFκΒ-pathway) compared with BCL11A, which indicates that the altered genes disrupting the NFκΒ pathway may be the driver genes of transformation rather than the previously suggested BCL11A. Moreover, a 17q21.33 amplification was exclusively found in tDLBCL, never in FL (P<0.04) or dnDLBCL, indicating an upregulation of genes of importance during the later phase of transformation. Taken together, our study demonstrates potential genomic markers for disease progression to clinically more aggressive forms. We also confirm the importance of the TP53-, CDKN2A-, and NFκΒ-pathways for the transformation from FL to DLBCL.

Details

Authors
  • Anna Kwiecinska
  • Koichi Ichimura
  • Mattias Berglund
  • Andrii Dinets
  • Luqman Sulaiman
  • V. Peter Collins
  • Catharina Larsson
  • Anna Porwit
  • Svetlana Bajalica Lagercrantz
External organisations
  • Karolinska University Hospital
  • Addenbrooke's Hospital
  • Uppsala University
  • University of Toronto
  • Toronto General Hospital
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Cancer and Oncology
  • Medical Genetics
Original languageEnglish
Pages (from-to)750-768
Number of pages19
JournalGenes Chromosomes and Cancer
Volume53
Issue number9
Publication statusPublished - 2014
Publication categoryResearch
Peer-reviewedYes
Externally publishedYes