β-amyloid peptides inhibit acetylcholine release from cholinergic presynaptic nerve endings isolated from an electric ray

Research output: Contribution to journalArticle

Abstract

We investigated the effects of β-amyloid (Aβ) peptides on cholinergic synaptosomes isolated from the electric organ of the Japanese marine ray Narke japonica. Fresh and pre-incubated solutions of Aβ(1-42) inhibited acetylcholine (ACh) release from the synaptosomes evoked by high [K+] depolarization when incubated with synaptosomes for 10 min before the depolarizing stimulus. A freshly prepared solution of Aβ(1-40) did not inhibit the evoked ACh release, but prolonged pre-incubation of Aβ(1-40) solution caused the inhibition. Aβ(1-15) neither in fresh nor pre-incubated solution inhibited. These results have demonstrated that Aβ peptides can acutely inhibit the depolarization-evoked release of ACh by acting directly on cholinergic presynaptic nerve endings. The electrophoresis analysis showed a strong correlation between Aβ aggregation and its inhibition for ACh release.

Details

Authors
  • Yasushi Satoh
  • Yutaka Hirakura
  • Sotaro Shibayama
  • Naohide Hirashima
  • Toshiharu Suzuki
  • Yutaka Kirino
External organisations
  • University of Tokyo
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Neurosciences

Keywords

  • β-Amyloid, aggregation, cholinergic neurons, electric organ, high [K] depolarization, nerve terminals, synaptosomes
Original languageEnglish
Pages (from-to)97-100
Number of pages4
JournalNeuroscience Letters
Volume302
Issue number2-3
Publication statusPublished - 2001 Apr 20
Publication categoryResearch
Peer-reviewedYes
Externally publishedYes