An adaptive alignment algorithm for quality-controlled label-free LC-MS.

Research output: Contribution to journalArticle

Abstract

Label-free quantification using precursor-based intensities is a versatile workflow for large-scale proteomics studies. The method requires extensive computational analysis and is therefore in need of robust quality control during the data mining stage. We present a new label-free data analysis workflow integrated into a multi-user software platform. A novel adaptive alignment algorithm has been developed to minimize the possible systematic bias introduced into the analysis. Parameters are estimated on the fly from the data at hand, producing a user-friendly analysis suite. Quality metrics are output in every step of the analysis as well as actively incorporated into the parameter estimation. We furthermore show the improvement of this system by comprehensive comparison to classical label-free analysis methodology as well as current state-of-the-art software.

Details

Authors
  • Marianne Sandin
  • Ashfaq Ali
  • Karin Hansson
  • Olle Månsson
  • Erik Andreasson
  • Svante Resjö
  • Fredrik Levander
Organisations
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Endocrinology and Diabetes
Original languageEnglish
Pages (from-to)1407-1420
JournalMolecular & Cellular Proteomics
Volume12
Issue number5
Publication statusPublished - 2013
Publication categoryResearch
Peer-reviewedYes