An alternative method for calibration of flow field flow fractionation channels for hydrodynamic radius determination: The nanoemulsion method (featuring multi angle light scattering)

Research output: Contribution to journalArticle

Abstract

This study suggests a novel method for determination of the channel height in asymmetrical flow field-flow fractionation (AF4), which can be used for calibration of the channel for hydrodynamic radius determinations. The novel method uses an oil-in-water nanoemulsion together with multi angle light scattering (MALS) and elution theory to determine channel height from an AF4 experiment. The method is validated using two orthogonal methods; first, by using standard particle elution experiments and, secondly, by imaging an assembled and carrier liquid filled channel by x-ray computed tomography (XCT). It is concluded that the channel height can be determined with approximately the same accuracy as with the traditional channel height determination technique. However, the nanoemulsion method can be used under more challenging conditions than standard particles, as the nanoemulsion remains stable in a wider pH range than the previously used standard particles. Moreover, the novel method is also more cost effective.

Details

Authors
Organisations
External organisations
  • Kristianstad University
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Fluid Mechanics and Acoustics

Keywords

  • AF4, Channel height, Flow field-flow fractionation, Multi angle light scattering, Nanoemulsion, X-ray computed tomography
Original languageEnglish
Pages (from-to)155-163
Number of pages9
JournalJournal of Chromatography A
Volume1533
Publication statusPublished - 2018 Jan 19
Publication categoryResearch
Peer-reviewedYes

Related infrastructure

View all (1)