An artifact in measurements of in vivo light-induced absorbance changes

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Abstract

The effects of scattered actinic radiation on photomultipliers (Hamamatsu R-562) were investigated. Using cotton-wool to model dense biological preparations, it was found that the scattered actinic radiation received by the photomultiplier gives rise to phytochrome-like signals. This demonstrated the necessity to shield the photomultiplier from scattered actinic light for sensitive measurements with light-scattering preparations.

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Subject classification (UKÄ) – MANDATORY

  • Biological Sciences
Original languageEnglish
Pages (from-to)271-274
JournalJournal of Biochemical and Biophysical Methods
Volume8
Issue number4
Publication statusPublished - 1983
Publication categoryResearch
Peer-reviewedYes