An artifact in measurements of in vivo light-induced absorbance changes

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An artifact in measurements of in vivo light-induced absorbance changes. / Hsiao, K. C.; Björn, Lars Olof.

In: Journal of Biochemical and Biophysical Methods, Vol. 8, No. 4, 1983, p. 271-274.

Research output: Contribution to journalArticle

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TY - JOUR

T1 - An artifact in measurements of in vivo light-induced absorbance changes

AU - Hsiao, K. C.

AU - Björn, Lars Olof

PY - 1983

Y1 - 1983

N2 - The effects of scattered actinic radiation on photomultipliers (Hamamatsu R-562) were investigated. Using cotton-wool to model dense biological preparations, it was found that the scattered actinic radiation received by the photomultiplier gives rise to phytochrome-like signals. This demonstrated the necessity to shield the photomultiplier from scattered actinic light for sensitive measurements with light-scattering preparations.

AB - The effects of scattered actinic radiation on photomultipliers (Hamamatsu R-562) were investigated. Using cotton-wool to model dense biological preparations, it was found that the scattered actinic radiation received by the photomultiplier gives rise to phytochrome-like signals. This demonstrated the necessity to shield the photomultiplier from scattered actinic light for sensitive measurements with light-scattering preparations.

U2 - 10.1016/0165-022X(83)90001-5

DO - 10.1016/0165-022X(83)90001-5

M3 - Article

VL - 8

SP - 271

EP - 274

JO - Journal of Proteomics

JF - Journal of Proteomics

SN - 1874-3919

IS - 4

ER -