An ELISA for grancalcin, a novel cytosolic calcium-binding protein present in leukocytes

Research output: Contribution to journalArticle

Abstract

Grancalcin is a newly discovered cytosolic calcium-binding protein, belonging to the group of EF-hand proteins. Grancalcin is specifically associated with cells originating in the bone marrow. Grancalcin binds reversibly to secretory vesicles and plasma membranes in human neutrophils and might therefore play a role in the regulation of vesicle/granule exocytosis. We describe here the production of recombinant grancalcin, the generation of antibodies to the protein and the development of a specific, accurate and sensitive ELISA for the detection of human leukocytic grancalcin. This ELISA may be useful for monitoring leukocyte infiltration into tissues.

Details

Authors
  • K Lollike
  • O Sørensen
  • J R Bundgaard
  • A W Segal
  • A Boyhan
  • N Borregaard
External organisations
  • Copenhagen University Hospital
  • University College London
Research areas and keywords

Keywords

  • Antibody Specificity, Calcium-Binding Proteins/analysis, Enzyme-Linked Immunosorbent Assay, Humans, Leukocytes, Mononuclear/chemistry, Neutrophils/chemistry, Recombinant Proteins
Original languageEnglish
Pages (from-to)1-8
Number of pages8
JournalJournal of Immunological Methods
Volume185
Issue number1
Publication statusPublished - 1995 Sep 11
Publication categoryResearch
Peer-reviewedYes
Externally publishedYes