An ELISA for grancalcin, a novel cytosolic calcium-binding protein present in leukocytes

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Bibtex

@article{7db97644bf47442eaf6a01ff78c31be5,
title = "An ELISA for grancalcin, a novel cytosolic calcium-binding protein present in leukocytes",
abstract = "Grancalcin is a newly discovered cytosolic calcium-binding protein, belonging to the group of EF-hand proteins. Grancalcin is specifically associated with cells originating in the bone marrow. Grancalcin binds reversibly to secretory vesicles and plasma membranes in human neutrophils and might therefore play a role in the regulation of vesicle/granule exocytosis. We describe here the production of recombinant grancalcin, the generation of antibodies to the protein and the development of a specific, accurate and sensitive ELISA for the detection of human leukocytic grancalcin. This ELISA may be useful for monitoring leukocyte infiltration into tissues.",
keywords = "Antibody Specificity, Calcium-Binding Proteins/analysis, Enzyme-Linked Immunosorbent Assay, Humans, Leukocytes, Mononuclear/chemistry, Neutrophils/chemistry, Recombinant Proteins",
author = "K Lollike and O S{\o}rensen and Bundgaard, {J R} and Segal, {A W} and A Boyhan and N Borregaard",
year = "1995",
month = sep,
day = "11",
doi = "10.1016/0022-1759(95)00099-v",
language = "English",
volume = "185",
pages = "1--8",
journal = "Journal of Immunological Methods",
issn = "1872-7905",
publisher = "Elsevier",
number = "1",

}