An ELISA for grancalcin, a novel cytosolic calcium-binding protein present in leukocytes

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An ELISA for grancalcin, a novel cytosolic calcium-binding protein present in leukocytes. / Lollike, K; Sørensen, O; Bundgaard, J R; Segal, A W; Boyhan, A; Borregaard, N.

In: Journal of Immunological Methods, Vol. 185, No. 1, 11.09.1995, p. 1-8.

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Lollike, K ; Sørensen, O ; Bundgaard, J R ; Segal, A W ; Boyhan, A ; Borregaard, N. / An ELISA for grancalcin, a novel cytosolic calcium-binding protein present in leukocytes. In: Journal of Immunological Methods. 1995 ; Vol. 185, No. 1. pp. 1-8.

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TY - JOUR

T1 - An ELISA for grancalcin, a novel cytosolic calcium-binding protein present in leukocytes

AU - Lollike, K

AU - Sørensen, O

AU - Bundgaard, J R

AU - Segal, A W

AU - Boyhan, A

AU - Borregaard, N

PY - 1995/9/11

Y1 - 1995/9/11

N2 - Grancalcin is a newly discovered cytosolic calcium-binding protein, belonging to the group of EF-hand proteins. Grancalcin is specifically associated with cells originating in the bone marrow. Grancalcin binds reversibly to secretory vesicles and plasma membranes in human neutrophils and might therefore play a role in the regulation of vesicle/granule exocytosis. We describe here the production of recombinant grancalcin, the generation of antibodies to the protein and the development of a specific, accurate and sensitive ELISA for the detection of human leukocytic grancalcin. This ELISA may be useful for monitoring leukocyte infiltration into tissues.

AB - Grancalcin is a newly discovered cytosolic calcium-binding protein, belonging to the group of EF-hand proteins. Grancalcin is specifically associated with cells originating in the bone marrow. Grancalcin binds reversibly to secretory vesicles and plasma membranes in human neutrophils and might therefore play a role in the regulation of vesicle/granule exocytosis. We describe here the production of recombinant grancalcin, the generation of antibodies to the protein and the development of a specific, accurate and sensitive ELISA for the detection of human leukocytic grancalcin. This ELISA may be useful for monitoring leukocyte infiltration into tissues.

KW - Antibody Specificity

KW - Calcium-Binding Proteins/analysis

KW - Enzyme-Linked Immunosorbent Assay

KW - Humans

KW - Leukocytes, Mononuclear/chemistry

KW - Neutrophils/chemistry

KW - Recombinant Proteins

U2 - 10.1016/0022-1759(95)00099-v

DO - 10.1016/0022-1759(95)00099-v

M3 - Article

C2 - 7665891

VL - 185

SP - 1

EP - 8

JO - Journal of Immunological Methods

JF - Journal of Immunological Methods

SN - 1872-7905

IS - 1

ER -