An ELISA for hCAP-18, the cathelicidin present in human neutrophils and plasma

Research output: Contribution to journalArticle

Abstract

hCAP-18 is a newly described protein of human neutrophilic granulocytes which belongs to the cathelicidin family of antimicrobial proteins. Members of this protein family share a common N-terminal sequence followed by a highly diverse antimicrobial, cationic C-terminus. The present work describes the production of recombinant hCAP-18, the generation of antibodies to the protein and the development of an accurate, sensitive and specific ELISA for the detection of hCAP-18 in cells, plasma and urine with a detection limit of 0.084 ng/ml. The amount of hCAP-18 in neutrophils is 0.627 microgram protein per 10(6) cells. The plasma level is 1.18 micrograms/ml which is several fold higher than for other neutrophil specific granule proteins. hCAP-18 is present in plasma as high molecular weight complexes. In accordance with this, hCAP-18 is barely excreted in the urine. The bone marrow appears to be the major source of plasma hCAP-18. The high level of hCAP-18 in plasma may provide an important defense against microorganisms and endotoxins.

Details

Authors
External organisations
  • Copenhagen University Hospital
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Immunology in the medical area

Keywords

  • Animals, Anti-Infective Agents/blood, Antibodies/chemistry, Antimicrobial Cationic Peptides, Carrier Proteins/blood, Cathelicidins, Enzyme-Linked Immunosorbent Assay/methods, Humans, Neutrophils/chemistry, Plasma/chemistry, Rabbits, Reproducibility of Results, Sensitivity and Specificity
Original languageEnglish
Pages (from-to)53-59
Number of pages7
JournalJournal of Immunological Methods
Volume206
Issue number1-2
Publication statusPublished - 1997 Aug 7
Publication categoryResearch
Peer-reviewedYes
Externally publishedYes