An UPLC-MS/MS assay to measure glutathione as marker for oxidative stress in cultured cells

Research output: Contribution to journalArticle


Oxidative stress plays a role in the onset and progression of a number of diseases, such as Alzheimer’s disease, diabetes and cancer, as well as ageing. Oxidative stress is caused by an increased production of reactive oxygen species and reduced antioxidant activity, resulting in the oxidation of glutathione. The ratio of reduced to oxidised glutathione is often used as a marker of the redox state in the cell. Whereas a variety of methods have been developed to measure glutathione in blood samples, methods to measure glutathione in cultured cells are scarce. Here we present a protocol to measure glutathione levels in cultured human and yeast cells using ultra-performance liquid chromatography-tandem mass spectrometry (UPLC–MS/MS).


  • Katharina Herzog
  • Lodewijk Ijlst
  • Arno G. van Cruchten
  • Carlo W.T. van Roermund
  • Wim Kulik
  • Ronald J.A. Wanders
  • Hans R. Waterham
External organisations
  • Academic Medical Center of University of Amsterdam (AMC)
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Cell and Molecular Biology


  • Cultured cells, Glutathione, Mass spectrometry, Oxidative stress, Yeast
Original languageEnglish
Article number45
Issue number3
Publication statusPublished - 2019 Mar 5
Publication categoryResearch