Anaesthetics and the blood vessel wall. Actions of propofol and sevoflurane on sympathetic and endothelial control of smooth muscle function.

Research output: ThesisDoctoral Thesis (compilation)

Abstract

The anaesthetics we use today dose-dependently decrease the mean arterial pressure partly due to direct or indirect effects on the blood vessels. In the present thesis human omental arteries and veins and rat femoral arteries were investigated in vitro concerning the effects of the intravenous anaesthetic propofol and the volatile anaesthetic sevoflurane on the function of the perivascular sympathetic nerves and the endothelial cells.

The effects of propofol on the kinetics of the neuronal uptake of noradrenaline were studied in the rat femoral artery. At lower propofol concentrations we found a decrease in the affinity of the noradrenaline transporters, which results in an uptake inhibition. At higher propofol concentrations this inhibition is counteracted by an increase in the efficiency of the uptake.

The effects of propofol on endothelium-dependent relaxation (induced by substance P) were studied in human omental arteries and veins. Propofol, at a clinically relevant concentration, was found to promote endothelium-dependent relaxation mediated via hyperpolarization in human omental arteries and via both nitric oxide and hyperpolarization in human omental veins.

The effects of sevoflurane on sympathetic neurotransmission were studied in human omental arteries and veins. We found that sevoflurane depresses the sympathetic neuromuscular transmission by lowering the neuronal noradrenaline release and noradrenaline sensitivity in the arteries and by lowering the noradrenaline release in the veins.

The effects of sevoflurane on endothelium-dependent relaxation (induced by substance P) were studied in human omental arteries and veins. Sevoflurane was found to promote endothelium-dependent relaxation in human omental arteries and veins probably via an enhancement of the response of smooth muscle cells to relaxing mediators.

Details

Authors
  • Karin Thorlacius
Organisations
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Anesthesiology and Intensive Care

Keywords

  • Anaesthesiology, intensive care, Anestesiologi, intensivvård, vein., artery, endothelium, sympathetic nervous system, inhalation agent sevoflurane, Anaesthesia, intravenous propofol
Original languageEnglish
QualificationDoctor
Awarding Institution
Supervisors/Assistant supervisor
  • [unknown], [unknown], Supervisor, External person
Award date2002 May 11
Publisher
  • Department of Anaesthesiology and Intensive Care, Lund
Print ISBNs91-628-5216-7
Publication statusPublished - 2002
Publication categoryResearch

Bibliographic note

Defence details Date: 2002-05-11 Time: 10:15 Place: Wallenberg Neurocenter, Lund External reviewer(s) Name: Lambert, David G. Title: [unknown] Affiliation: Anaesthetic Pharmacology, Leicester, UK --- Article: Sandström K, Wallerstedt SM, Törnebrandt K, Bodelsson M: Effects of propofol on desipramine-sensitive [3H]-noradrenaline uptake kinetics in rat femoral artery. Acta Anaesthesiol Scand 2000; 44:1011-1016. Article: Bodelsson G, Sandström K, Wallerstedt SM, Hidestål J, Törnebrandt K, Bodelsson M: Effects of propofol on substance P-induced relaxation in isolated human omental arteries and veins. Eur J Anaesthesiol 2000; 17:720-728. Article: Thorlacius K, Zhou Jun C, Bodelsson M: Effects of sevoflurane on sympathetic neurotransmission in human omental arteries and veins. Submitted. Article: Thorlacius K, Bodelsson M: Sevoflurane promotes endothelium-dependent smooth muscle relaxation in isolated human omental arteries and veins. Submitted.