Analysis of bacterial surface interactions with mass spectrometry-based proteomics

Research output: Chapter in Book/Report/Conference proceedingBook chapter

Abstract

Host–pathogen protein–protein interaction networks are highly complex and dynamic. In this experimental protocol we describe a method to isolate host proteins attached to the bacterial surface followed by quantitative mass spectrometry based proteomics analysis. This technique provides an overview of the host–pathogen interaction network, which can be used to guide directed perturbations of the system, and to select target of specific interest for further studies.

Details

Authors
Organisations
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Cell and Molecular Biology
  • Bioinformatics and Systems Biology

Keywords

  • Bacteria, Bioinformatics, Mass spectrometry, Peptide solid phase extraction, Proteomics, Surface absorption, Trypsin digestion
Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press
Pages17-24
Number of pages8
Volume1535
Publication statusPublished - 2017
Publication categoryResearch
Peer-reviewedYes

Publication series

NameMethods in Molecular Biology
Volume1535
ISSN (Print)10643745