Analysis of vesicular trafficking in primary neurons by live imaging.

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Abstract

Alzheimer's disease, the most common neurodegenerative disease, is characterized by a progressive loss of synapses and accumulation of amyloid-beta (Aβ) peptides in the brain. Previous studies demonstrated that acute increase in synaptic activity in cultured hippocampal slices and mouse brains (Cirrito et al. Neuron 48: 913-922, 2005; Kamenetz et al. Neuron 37: 925-937, 2003) enhanced secretion of Aβ. Since synaptic activity promotes Aβ secretion, it could also affect the trafficking and processing of its precursor, the amyloid precursor protein (APP). Here, we describe a method to investigate the effect of acute synaptic activation on APP trafficking within dendrites.

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  • Neurosciences
Original languageEnglish
Pages (from-to)343-350
JournalMethods in Molecular Biology
Volume793
Publication statusPublished - 2011
Publication categoryResearch
Peer-reviewedYes