Antibody immobilization strategy for the development of a capacitive immunosensor detecting zearalenone

Research output: Contribution to journalArticle


title = "Antibody immobilization strategy for the development of a capacitive immunosensor detecting zearalenone",
abstract = "A highly sensitive flow-injection capacitive immunosensor was developed for detection of the mycotoxin zearalenone (ZEN). Different strategies for immobilization of an anti-ZEN antibody on the surface of a gold electrode, i.e. polytyramine or self-assembled monolayers (SAMs) of 3-mercaptopropionic acid (3-MPA) and lipoic acid (LA), were used and their performances were compared. The LA- and 3-MPA-based systems showed broad linear ranges for ZEN determination, i.e. from 0.010 nM to 10 nM and from 0.020 nM to 10 nM, respectively. Under optimal conditions, the LA-based immunosensor was capable of performing up till 13 regeneration-interaction cycles (with use of glycine HCl, pH 2.4) with a limit of detection (LOD) of 0.0060 nM, equivalent to 1.9 pg mL−1. It also demonstrated a good inter-assay precision (RSD < 10{\%}). However, the tyramine-based capacitive immunosensor showed a bad repeatability (only 4 regeneration-interaction cycles were possible) and inter-assay precision (RSD > 15{\%}) which did not allow sensitive and precise measurements. The LA-based method was compared with a direct ELISA. These results demonstrated that the label-free developed capacitive immunosensor had a better sensitivity and shorter analysis time in comparison with the direct microwell-plate format.",
keywords = "Capacitive immunosensor, Mycotoxin, Polytyramine, Self-assembled monolayers, Zearalenone",
author = "Astrid Foubert and Beloglazova, {Natalia V.} and Martin Hedstr{\"o}m and {De Saeger}, Sarah",
year = "2019",
month = "1",
day = "1",
doi = "10.1016/j.talanta.2018.08.062",
language = "English",
volume = "191",
pages = "202--208",
journal = "Talanta",
issn = "1873-3573",
publisher = "Elsevier",