Antibody immobilization strategy for the development of a capacitive immunosensor detecting zearalenone

Research output: Contribution to journalArticle

Standard

Antibody immobilization strategy for the development of a capacitive immunosensor detecting zearalenone. / Foubert, Astrid; Beloglazova, Natalia V.; Hedström, Martin; De Saeger, Sarah.

In: Talanta, Vol. 191, 01.01.2019, p. 202-208.

Research output: Contribution to journalArticle

Harvard

APA

CBE

MLA

Vancouver

Author

Foubert, Astrid ; Beloglazova, Natalia V. ; Hedström, Martin ; De Saeger, Sarah. / Antibody immobilization strategy for the development of a capacitive immunosensor detecting zearalenone. In: Talanta. 2019 ; Vol. 191. pp. 202-208.

RIS

TY - JOUR

T1 - Antibody immobilization strategy for the development of a capacitive immunosensor detecting zearalenone

AU - Foubert, Astrid

AU - Beloglazova, Natalia V.

AU - Hedström, Martin

AU - De Saeger, Sarah

PY - 2019/1/1

Y1 - 2019/1/1

N2 - A highly sensitive flow-injection capacitive immunosensor was developed for detection of the mycotoxin zearalenone (ZEN). Different strategies for immobilization of an anti-ZEN antibody on the surface of a gold electrode, i.e. polytyramine or self-assembled monolayers (SAMs) of 3-mercaptopropionic acid (3-MPA) and lipoic acid (LA), were used and their performances were compared. The LA- and 3-MPA-based systems showed broad linear ranges for ZEN determination, i.e. from 0.010 nM to 10 nM and from 0.020 nM to 10 nM, respectively. Under optimal conditions, the LA-based immunosensor was capable of performing up till 13 regeneration-interaction cycles (with use of glycine HCl, pH 2.4) with a limit of detection (LOD) of 0.0060 nM, equivalent to 1.9 pg mL−1. It also demonstrated a good inter-assay precision (RSD < 10%). However, the tyramine-based capacitive immunosensor showed a bad repeatability (only 4 regeneration-interaction cycles were possible) and inter-assay precision (RSD > 15%) which did not allow sensitive and precise measurements. The LA-based method was compared with a direct ELISA. These results demonstrated that the label-free developed capacitive immunosensor had a better sensitivity and shorter analysis time in comparison with the direct microwell-plate format.

AB - A highly sensitive flow-injection capacitive immunosensor was developed for detection of the mycotoxin zearalenone (ZEN). Different strategies for immobilization of an anti-ZEN antibody on the surface of a gold electrode, i.e. polytyramine or self-assembled monolayers (SAMs) of 3-mercaptopropionic acid (3-MPA) and lipoic acid (LA), were used and their performances were compared. The LA- and 3-MPA-based systems showed broad linear ranges for ZEN determination, i.e. from 0.010 nM to 10 nM and from 0.020 nM to 10 nM, respectively. Under optimal conditions, the LA-based immunosensor was capable of performing up till 13 regeneration-interaction cycles (with use of glycine HCl, pH 2.4) with a limit of detection (LOD) of 0.0060 nM, equivalent to 1.9 pg mL−1. It also demonstrated a good inter-assay precision (RSD < 10%). However, the tyramine-based capacitive immunosensor showed a bad repeatability (only 4 regeneration-interaction cycles were possible) and inter-assay precision (RSD > 15%) which did not allow sensitive and precise measurements. The LA-based method was compared with a direct ELISA. These results demonstrated that the label-free developed capacitive immunosensor had a better sensitivity and shorter analysis time in comparison with the direct microwell-plate format.

KW - Capacitive immunosensor

KW - Mycotoxin

KW - Polytyramine

KW - Self-assembled monolayers

KW - Zearalenone

U2 - 10.1016/j.talanta.2018.08.062

DO - 10.1016/j.talanta.2018.08.062

M3 - Article

VL - 191

SP - 202

EP - 208

JO - Talanta

T2 - Talanta

JF - Talanta

SN - 1873-3573

ER -