Antifungal activity of C3a and C3a-derived peptides against Candida.

Research output: Contribution to journalArticle

Abstract

Antimicrobial peptides are generated during activation of the complement system [Nordahl et al. Proc. Nat1. Acad. Sci. U. S. A. 2004, 101:16879-16884]. Here we show that the anaphylatoxin C3a exerts antimicrobial effects against the yeast Candida. Fluorescence microscopy and electron microscopy analysis demonstrated that C3a-derived peptides bound to the cell surface of Candida, and induced membrane perturbations and release of extracellular material. Various Candida isolates were found to induce complement degradation, leading to generation of C3a. Arginine residues were found to be critical for the antifungal and membrane breaking activity of a C3a-derived antimicrobial peptide, CNY21 (C3a; Cys(57)-Arg(77)). A CNY21 variant with increased positive net charge displayed enhanced antifungal activity. Thus, C3a-derived peptides can be utilized as templates in the development of peptide-based antifungal therapies. (c) 2006 Elsevier B.V All rights reserved.

Details

Authors
Organisations
External organisations
  • Uppsala University
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Other Clinical Medicine

Keywords

  • atopic dermatitis, Candida, innate immunity, antimicrobial peptides, complement, structure-activity relationship study
Original languageEnglish
Pages (from-to)346-353
JournalBiochimica et Biophysica Acta - Biomembranes
Volume1768
Issue number2
Publication statusPublished - 2007
Publication categoryResearch
Peer-reviewedYes

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