Antimicrobial activity of human seminal plasma and seminal plasma proteins

Research output: ThesisDoctoral Thesis (compilation)

Abstract

Seminal plasma is semen without the spermatozoa. Human seminal plasma is a complex mixture of secretions from the sex accessory glands, mainly the seminal vesicles and the prostate. Seminal plasma has high protein content and it also contains ions, sugars and low molecular weight components. Immediately after ejaculation the semenogelins and fibronectin aggregate and a coagulum forms trapping the spermatozoa. Seminal plasma is then liquefied as the semenogelins are degraded by prostate specific antigen and the spermatozoa are released. We found that the peptides formed when the semenogelins are degraded were responsible for the major bactericidal activity of seminal plasma. The activity of these peptides was strictly zinc-dependent. We also found potent antifungal activity in seminal unleashed by the acid vaginal pH. The antifungal activity was mediated by beta-microseminoprotein (MSP). The activity of MSP mapped to a region in the C-terminal part of the protein. The antifungal activity was inhibited by calcium binding to MSP at neutral pH but not at the acid vaginal pH explaining the pH-dependent antifungal activity of seminal plasma. This is the first report of antifungal activity of seminal plasma activity and represents novel mechanism of regulation of antifungal activity. We also found that the seminal plasma protein, CRISP-3, possibly had an antifungal activity regulated in pH-dependent manner demonstrating that pH may be a important factor to consider when studying the antimicrobial activity of seminal plasma proteins .

Details

Authors
  • Anneli Edström
Organisations
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Infectious Medicine

Keywords

  • semenogelin, antibacterial peptides, antifungal peptides, seminal plasma, antimicrobial peptides, vaginal pH, beta-microseminoprotein, peptides
Original languageEnglish
QualificationDoctor
Awarding Institution
Supervisors/Assistant supervisor
Award date2010 Oct 29
Publisher
  • Lund University
Print ISBNs978-91-86671-16-7
Publication statusPublished - 2010
Publication categoryResearch

Bibliographic note

Defence details Date: 2010-10-29 Time: 09:15 Place: Belfragesalen, BMC External reviewer(s) Name: Schröder, Jens Michael Title: PhD Affiliation: Department of Dermatology, University of Kiel, Germany ---

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Related research output

Edström, A. M. L., Johan Malm, Frohm, B., Martellini, J. A., Aleksander Giwercman, Mörgelin, M., Cole, A. M. & Ole E Sørensen, 2008, In : Journal of immunology (Baltimore, Md. : 1950). 181, 5, p. 3413-3421

Research output: Contribution to journalArticle

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