Apoptosis-inducing factor mediates dopaminergic cell death in response to lps-induced inflammatory stimulus Evidence in Parkinson's disease patients.

Research output: Contribution to journalArticle

Bibtex

@article{e7bf7ebc6d864bff80252bfedfde3e22,
title = "Apoptosis-inducing factor mediates dopaminergic cell death in response to lps-induced inflammatory stimulus Evidence in Parkinson's disease patients.",
abstract = "We show that intranigral lipopolysaccharide (LPS) injection, which provokes specific degeneration of DA neurons, induced caspase-3 activation in the rat ventral mesencephalon, which was mostly associated with glial cells. In contrast, nigral DA neurons exhibited AIF nuclear translocation in response to LPS. A significant decrease of the Bcl-2/Bax ratio in nigral tissue after LPS injection was observed. We next developed an in vitro co-culture system with the microglial BV2 and the DA neuronal MN9D murine cell lines. The silencing of caspase-3 or AIF by small interfering RNAs exclusively in the DA MN9D cells demonstrated the key role of AIF in the LPS-induced death of DA cells. In vivo chemical inhibition of caspases and poly(ADP-ribose)polymerase-1, an upstream regulator of AIF release and calpain, proved the central role of the AIF-dependent pathway in LPS-induced nigral DA cell death. We also observed nuclear translocation of AIF in the ventral mesencephalon of Parkinson's disease subjects.",
author = "{Burguillos Garcia}, Miguel and N Hajji and E Englund and A Persson and {Cenci Nilsson}, Angela and A Machado and J Cano and B Joseph and Venero, {J L}",
year = "2011",
doi = "10.1016/j.nbd.2010.09.005",
language = "English",
volume = "41",
pages = "177--188",
journal = "Neurobiology of Disease",
issn = "0969-9961",
publisher = "Elsevier",

}