Applications of optical DNA mapping in microbiology

Research output: Contribution to journalReview article

Abstract

Optical mapping (OM) has been used in microbiology for the past 20 years, initially as a technique to facilitate DNA sequence-based studies; however, with decreases in DNA sequencing costs and increases in sequence output from automated sequencing platforms, OM has grown into an important auxiliary tool for genome assembly and comparison. Currently, there are a number of new and exciting applications for OM in the field of microbiology, including investigation of disease outbreaks, identification of specific genes of clinical and/or epidemiological relevance, and the possibility of single-cell analysis when combined with cell-sorting approaches. In addition, designing lab-on-a-chip systems based on OM is now feasible and will allow the integrated and automated microbiological analysis of biological fluids. Here, we review the basic technology of OM, detail the current state of the art of the field, and look ahead to possible future developments in OM technology for microbiological applications.

Details

Authors
Organisations
External organisations
  • Chalmers University of Technology
  • Catholic University of Portugal
  • University of Porto
  • University of the Balearic Islands
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Microbiology
  • Medical Laboratory and Measurements Technologies

Keywords

  • DNA-based identification, Imaging technique, Lab-on-a chip, Microbiological clinical diagnosis, Optical DNA mapping
Original languageEnglish
Pages (from-to)255-267
Number of pages13
JournalBioTechniques
Volume62
Issue number6
Publication statusPublished - 2017
Publication categoryResearch
Peer-reviewedYes