Aqueous two-phase partitioning for proteomic monitoring of cell surface biomarkers in human peripheral blood mononuclear cells

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Abstract

For proteomic monitoring of processes such as allergy or inflammation an efficient pre-fractionation strategy is required. We isolated plasma membranes from human peripheral blood mononuclear (PBM) cells by aqueous two-phase partitioning. After 1DE combined with LC-MS/MS, several cell surface marker proteins and in total 60 different plasma membrane proteins (out of 84 identified proteins, i.e., 72%) were detected. Plasma membranes obtained were from only one human donor, the procedure is therefore applicable for individual patient screening.

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Subject classification (UKÄ) – MANDATORY

  • Biological Sciences

Keywords

  • proteomics, prefractionation, two-phase partitioning, aqueous, cell surface biomarkers, allergy, plasma membrane proteins
Original languageEnglish
Pages (from-to)1168-1175
JournalJournal of Proteome Research
Volume5
Issue number5
Publication statusPublished - 2006
Publication categoryResearch
Peer-reviewedYes