Arachidonic acid absorption in human jejunum in organ culture: effects of ethanol

Research output: Contribution to journalArticle

Abstract

Human jejunal tissue obtained by peroral biopsy was cultured in control medium and in medium containing 100 mmol l-1 ethanol. Subsequently, the incorporation and metabolism of [3H]-arachidonic and [14C]-linoleic acid were evaluated. Of the two fatty acids a significantly higher amount of [3H]-arachidonic acid was incorporated into phospholipids and more [14C]-linoleic acid was incorporated into triacylglycerols. This preferential distribution of the labelled fatty acids was not affected by ethanol, but when ethanol was present in the tissue culture medium, there was a significant decrease in the incorporation of both fatty acids into tissue phospholipids. The study thus shows that ethanol in moderate concentrations can affect human jejunal absorption and metabolism of polyenoic fatty acids, contributing to a decrease in the amount of eicosanoid precursors available in jejunal tissue.

Details

Authors
Organisations
External organisations
  • Skåne University Hospital
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Pharmacology and Toxicology

Keywords

  • Arachidonic Acid, Arachidonic Acids/metabolism, Ethanol/pharmacology, Humans, Intestinal Absorption/drug effects, Intestinal Mucosa/drug effects, Jejunum/drug effects, Linoleic Acid, Linoleic Acids/metabolism, Organ Culture Techniques
Original languageEnglish
Pages (from-to)506-10
Number of pages5
JournalEuropean Journal of Clinical Investigation
Volume20
Issue number5
Publication statusPublished - 1990 Oct
Publication categoryResearch
Peer-reviewedYes