Assessing risks of Wolbachia DNA cross-specimens contamination following mass collection and ethanol storage

Research output: Contribution to journalArticle

Abstract

Wolbachia and other intracellular bacteria that manipulate reproduction are widespread and can have major consequences on the ecology and evolution of their hosts. Several studies have attempted to assess the host range of these bacteria based on polymerase chain reaction assays on material preserved and collected using a variety of methods. While collecting in the field, mass storage in ethanol before sorting specimens in the laboratory is by far the easiest technique, and an integral component of Malaise trapping. This implicitly relies on the assumption that mass ethanol storage does not produce cross‐contamination of Wolbachia DNA among specimens. Here we test this assumption. The absence of cross contamination between known positive and negative samples stored within a vial indicate there is no reason to believe collective storage of specimens creates artefactual increases in the incidence of Wolbachia or other intracellular bacteria.

Details

Authors
  • Anne Duplouy
  • Coralie Vermenot
  • Neil Davies
  • Georges K Roderick
  • Gregory DD Hurst
  • Sylvain Charlat
External organisations
  • The Center for Insular Research and Observatory of the Environment (CRIOBE)
  • University of California, Berkeley
  • University College London
  • University of Lyon
  • University of Queensland
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Ecology
Original languageEnglish
Pages (from-to)46-50
JournalMolecular Ecology Resources
Volume9
Issue number1
Publication statusPublished - 2009
Publication categoryResearch
Peer-reviewedYes
Externally publishedYes