Assignment of allotypes G1m(a+) and G1m(a-) at the genomic level by polymerase chain reaction analysis

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A method of assignment of the human immunoglobulin allotypes G1m(a+) and G1m(a-) without the use of serological reagents is described. It is based upon oligonucleotide-directed enzymatic amplification of genomic segments encoding CH3 of gamma chains, followed by dot-blot hybridization of radioactively labelled oligonucleotides to the amplified DNA. The method was used to classify the immunoglobulin allotypes of 11 persons, six G1m(a+) and five G1m(a-), and the resultant classification agreed completely with that of classical serological typing.


Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Pharmacology and Toxicology
  • Medicinal Chemistry
Original languageEnglish
Pages (from-to)205-212
JournalExperimental and Clinical Immunogenetics
Issue number4
Publication statusPublished - 1990
Publication categoryResearch