Assignment of gene for coagulation factor V to chromosome 1 in man and to chromosome 13 in rat

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Abstract

Two different factor V cDNA fragments were used as hydridization probes in the chromosomal assignment of the human and rat factor V genes. A 1.6-kb EcoRI fragment was used as a hybridization probe to analyze a panel of human-rodent somatic cell hybrids. Cosegregation of factor V specific DNA restriction fragments with human chromosome 1 was observed. In addition, a panel of rat-mouse somatic cell hybrids was analyzed with another human factor V cDNA probe to localize the gene for rat coagulation factor V. In the rat, the gene for coagulation factor V was found to be located in chromosome 13. This is the first gene in the rat to be localized to chromosome 13.

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Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Medicinal Chemistry

Keywords

  • DNA Probes, Molecular, Cloning, Pair 1, Human, *Chromosomes, *Chromosome Mapping, Animals, Southern, Blotting, Factor V/*genetics, Humans, Hybrid Cells, Nucleic Acid Hybridization, Rats, Research Support, Non-U.S. Gov't
Original languageEnglish
Pages (from-to)509-14
JournalSomat Cell Mol Genet
Volume14
Issue number5
Publication statusPublished - 1988
Publication categoryResearch
Peer-reviewedYes

Bibliographic note

5