Association of erythroid transcription factors: complexes involving the LIM protein RBTN2 and the zinc-finger protein GATA1

Research output: Contribution to journalArticle

Abstract

The RBTN2 LIM-domain protein, originally identified as an oncogenic protein in human T-cell leukemia, is essential for erythropoiesis. A possible role for RBTN2 in transcription during erythropoiesis has been investigated. Direct interaction of the RBTN2 protein was observed in vivo and in vitro with the GATA1 or -2 zinc-finger transcription factors, as well as with the basic helix-loop-helix protein TAL1. By using mammalian two-hybrid analysis, complexes involving RBTN2, TAL1, and GATA1, together with E47, the basic helix-loop-helix heterodimerization partner of TAL1, could be demonstrated. Thus, a molecular link exists between three proteins crucial for erythropoiesis, and the data suggest that variations in amounts of complexes involving RBTN2, TAL1, and GATA1 could be important for erythroid differentiation.

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Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Hematology

Keywords

  • Adaptor Proteins, Signal Transducing, Amino Acid Sequence, Animals, Basic Helix-Loop-Helix Transcription Factors, DNA-Binding Proteins, Erythroid-Specific DNA-Binding Factors, Erythropoiesis, GATA1 Transcription Factor, Helix-Loop-Helix Motifs, Homeodomain Proteins, LIM Domain Proteins, Leukemia, Erythroblastic, Acute, Metalloproteins, Mice, Molecular Sequence Data, Protein Binding, Proto-Oncogene Proteins, Recombinant Proteins, TCF Transcription Factors, Transcription Factor 7-Like 1 Protein, Transcription Factors, Tumor Cells, Cultured, Zinc Fingers
Original languageEnglish
Pages (from-to)9585-9
Number of pages5
JournalProceedings of the National Academy of Sciences
Volume92
Issue number21
Publication statusPublished - 1995 Oct 10
Publication categoryResearch
Peer-reviewedYes