Association of erythroid transcription factors: complexes involving the LIM protein RBTN2 and the zinc-finger protein GATA1

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Association of erythroid transcription factors : complexes involving the LIM protein RBTN2 and the zinc-finger protein GATA1. / Osada, H; Grutz, G; Axelson, H; Forster, A; Rabbitts, T H.

In: Proceedings of the National Academy of Sciences, Vol. 92, No. 21, 10.10.1995, p. 9585-9.

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TY - JOUR

T1 - Association of erythroid transcription factors

T2 - complexes involving the LIM protein RBTN2 and the zinc-finger protein GATA1

AU - Osada, H

AU - Grutz, G

AU - Axelson, H

AU - Forster, A

AU - Rabbitts, T H

PY - 1995/10/10

Y1 - 1995/10/10

N2 - The RBTN2 LIM-domain protein, originally identified as an oncogenic protein in human T-cell leukemia, is essential for erythropoiesis. A possible role for RBTN2 in transcription during erythropoiesis has been investigated. Direct interaction of the RBTN2 protein was observed in vivo and in vitro with the GATA1 or -2 zinc-finger transcription factors, as well as with the basic helix-loop-helix protein TAL1. By using mammalian two-hybrid analysis, complexes involving RBTN2, TAL1, and GATA1, together with E47, the basic helix-loop-helix heterodimerization partner of TAL1, could be demonstrated. Thus, a molecular link exists between three proteins crucial for erythropoiesis, and the data suggest that variations in amounts of complexes involving RBTN2, TAL1, and GATA1 could be important for erythroid differentiation.

AB - The RBTN2 LIM-domain protein, originally identified as an oncogenic protein in human T-cell leukemia, is essential for erythropoiesis. A possible role for RBTN2 in transcription during erythropoiesis has been investigated. Direct interaction of the RBTN2 protein was observed in vivo and in vitro with the GATA1 or -2 zinc-finger transcription factors, as well as with the basic helix-loop-helix protein TAL1. By using mammalian two-hybrid analysis, complexes involving RBTN2, TAL1, and GATA1, together with E47, the basic helix-loop-helix heterodimerization partner of TAL1, could be demonstrated. Thus, a molecular link exists between three proteins crucial for erythropoiesis, and the data suggest that variations in amounts of complexes involving RBTN2, TAL1, and GATA1 could be important for erythroid differentiation.

KW - Adaptor Proteins, Signal Transducing

KW - Amino Acid Sequence

KW - Animals

KW - Basic Helix-Loop-Helix Transcription Factors

KW - DNA-Binding Proteins

KW - Erythroid-Specific DNA-Binding Factors

KW - Erythropoiesis

KW - GATA1 Transcription Factor

KW - Helix-Loop-Helix Motifs

KW - Homeodomain Proteins

KW - LIM Domain Proteins

KW - Leukemia, Erythroblastic, Acute

KW - Metalloproteins

KW - Mice

KW - Molecular Sequence Data

KW - Protein Binding

KW - Proto-Oncogene Proteins

KW - Recombinant Proteins

KW - TCF Transcription Factors

KW - Transcription Factor 7-Like 1 Protein

KW - Transcription Factors

KW - Tumor Cells, Cultured

KW - Zinc Fingers

M3 - Article

VL - 92

SP - 9585

EP - 9589

JO - Proceedings of the National Academy of Sciences

JF - Proceedings of the National Academy of Sciences

SN - 1091-6490

IS - 21

ER -