Association of serum MiR-142-3p and MiR-101-3p levels with acute cellular rejection after heart transplantation

Research output: Contribution to journalArticle

Abstract

Background: Identifying non-invasive and reliable blood-derived biomarkers for early detection of acute cellular rejection in heart transplant recipients is of great importance in clinical practice. MicroRNAs are small molecules found to be stable in serum and their expression patterns reflect both physiological and underlying pathological conditions in human. Methods: We compared a group of heart transplant recipients with histologically-verified acute cellular rejection (ACR, n = 26) with a control group of heart transplant recipients without allograft rejection (NR, n = 37) by assessing the levels of a select set of microRNAs in serum specimens. Results: The levels of seven microRNAs, miR-142-3p, miR-101-3p, miR-424-5p, miR-27a-3p, miR-144-3p, miR-339-3p and miR-326 were significantly higher in ACR group compared to the control group and could discriminate between patients with and without allograft rejection. MiR-142-3p and miR-101-3p had the best diagnostic test performance among the microRNAs tested. Serum levels of miR-142-3p and miR-101-3p were independent of calcineurin inhibitor levels, as measured by tacrolimus and cyclosporin; kidney function, as measured by creatinine level, and general inflammation state, as measured by CRP level. Conclusion: This study demonstrated two microRNAs, miR-142-3p and miR-101-3p, that could be relevant as non-invasive diagnostic tools for identifying heart transplant patients with acute cellular rejection.

Details

Authors
  • Ihdina Sukma Dewi
  • Zsuzsanna Hollander
  • Karen K. Lam
  • Janet Wilson McManus
  • Scott J. Tebbutt
  • Raymond T. Ng
  • Paul A. Keown
  • Robert W. McMaster
  • Bruce M. McManus
  • Olof Gidlöf
  • Jenny Öhman
Organisations
External organisations
  • Skåne University Hospital
  • University of British Columbia
  • PROOF Centre for Excellence
  • Canadian Blood Services
  • Vancouver General Hospital
  • Vancouver Coastal Health Research Institute
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Clinical Laboratory Medicine
Original languageEnglish
Article numbere0170842
JournalPLoS ONE
Volume12
Issue number1
Publication statusPublished - 2017
Publication categoryResearch
Peer-reviewedYes

Related research output

Ihdina Sukma Dewi, 2017, (Submitted) Lund: Lund University: Faculty of Medicine.

Research output: ThesisDoctoral Thesis (compilation)

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