At low water activity alpha-chymotrypsin is more active in an ionic liquid than in non-ionic organic solvents

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Abstract

The kinetics of the alpha-chymotrypsin catalysed transesterification of N-acetyl-l-phenylalanine ethyl ester with 1-butanol and the competing hydrolysis were evaluated at fixed water activity in two ionic liquids and two non-ionic organic solvents. In most respects the four solvents behaved similarly. However, at a water activity of 0.33, higher catalytic activity was observed in the ionic liquid, 1-butyl-3-methylimidazolium bis[(trifluoromethyl)sulfonyl]amide, than in the other solvents, and at a(w)=0.11 catalysis was only observed in this solvent.

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Subject classification (UKÄ) – MANDATORY

  • Industrial Biotechnology

Keywords

  • water activity, alpha-chymotrypsin, ionic liquids
Original languageEnglish
Pages (from-to)867-872
JournalBiotechnology Letters
Volume24
Issue number11
Publication statusPublished - 2002
Publication categoryResearch
Peer-reviewedYes