Auto- and alloimmune reactivity to human islet allografts transplanted into type 1 diabetic patients

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Auto- and alloimmune reactivity to human islet allografts transplanted into type 1 diabetic patients. / Roep, Bart O.; Stobbe, Inge; Duinkerken, Gaby; Van Rood, Jon J.; Lernmark, Åke; Keymeulen, Bart; Pipeleers, Danny; Claas, Frans H.J.; De Vries, René R.P.

In: Diabetes, Vol. 48, No. 3, 01.01.1999, p. 484-490.

Research output: Contribution to journalArticle

Harvard

Roep, BO, Stobbe, I, Duinkerken, G, Van Rood, JJ, Lernmark, Å, Keymeulen, B, Pipeleers, D, Claas, FHJ & De Vries, RRP 1999, 'Auto- and alloimmune reactivity to human islet allografts transplanted into type 1 diabetic patients', Diabetes, vol. 48, no. 3, pp. 484-490. https://doi.org/10.2337/diabetes.48.3.484

APA

Roep, B. O., Stobbe, I., Duinkerken, G., Van Rood, J. J., Lernmark, Å., Keymeulen, B., Pipeleers, D., Claas, F. H. J., & De Vries, R. R. P. (1999). Auto- and alloimmune reactivity to human islet allografts transplanted into type 1 diabetic patients. Diabetes, 48(3), 484-490. https://doi.org/10.2337/diabetes.48.3.484

CBE

Roep BO, Stobbe I, Duinkerken G, Van Rood JJ, Lernmark Å, Keymeulen B, Pipeleers D, Claas FHJ, De Vries RRP. 1999. Auto- and alloimmune reactivity to human islet allografts transplanted into type 1 diabetic patients. Diabetes. 48(3):484-490. https://doi.org/10.2337/diabetes.48.3.484

MLA

Vancouver

Author

Roep, Bart O. ; Stobbe, Inge ; Duinkerken, Gaby ; Van Rood, Jon J. ; Lernmark, Åke ; Keymeulen, Bart ; Pipeleers, Danny ; Claas, Frans H.J. ; De Vries, René R.P. / Auto- and alloimmune reactivity to human islet allografts transplanted into type 1 diabetic patients. In: Diabetes. 1999 ; Vol. 48, No. 3. pp. 484-490.

RIS

TY - JOUR

T1 - Auto- and alloimmune reactivity to human islet allografts transplanted into type 1 diabetic patients

AU - Roep, Bart O.

AU - Stobbe, Inge

AU - Duinkerken, Gaby

AU - Van Rood, Jon J.

AU - Lernmark, Åke

AU - Keymeulen, Bart

AU - Pipeleers, Danny

AU - Claas, Frans H.J.

AU - De Vries, René R.P.

PY - 1999/1/1

Y1 - 1999/1/1

N2 - Allogeneic islet transplantation can restore an insulin-independent state in C-peptide-negative type 1 diabetic patients. We recently reported three cases of surviving islet allografts that were implanted in type 1 diabetic patients under maintenance immune suppression for a previous kidney graft. The present study compares islet graft-specific cellular auto- and alloreactivity in peripheral blood from those three recipients and from four patients with failing islet allografts measured over a period of 6 months after portal islet implantation. The three cases that remained C-peptide- positive for >1 year exhibited no signs of alloreactivity, and their autoreactivity to islet autoantigens was only marginally increased. In contrast, rapid failure (<3 weeks) in three other cases was accompanied by increases in precursor frequencies of graft-specific alloreactive T-cells; in one of them, the alloreactivity was preceded by a sharply increased autoreactivity to several islet autoantigens. One recipient had a delayed loss of islet graft function (33 weeks); he did not exhibit signs of graft- specific alloimmunity, but developed a delayed increase in autoreactivity. The parallel between metabolic outcome of human β-cell allografts and cellular auto- and alloreactivity in peripheral blood suggests a causal relationship. The present study therefore demonstrates that T-cell reactivities in peripheral blood can be used to monitor immune mechanisms, which influence survival of β-cell allografts in diabetic patients.

AB - Allogeneic islet transplantation can restore an insulin-independent state in C-peptide-negative type 1 diabetic patients. We recently reported three cases of surviving islet allografts that were implanted in type 1 diabetic patients under maintenance immune suppression for a previous kidney graft. The present study compares islet graft-specific cellular auto- and alloreactivity in peripheral blood from those three recipients and from four patients with failing islet allografts measured over a period of 6 months after portal islet implantation. The three cases that remained C-peptide- positive for >1 year exhibited no signs of alloreactivity, and their autoreactivity to islet autoantigens was only marginally increased. In contrast, rapid failure (<3 weeks) in three other cases was accompanied by increases in precursor frequencies of graft-specific alloreactive T-cells; in one of them, the alloreactivity was preceded by a sharply increased autoreactivity to several islet autoantigens. One recipient had a delayed loss of islet graft function (33 weeks); he did not exhibit signs of graft- specific alloimmunity, but developed a delayed increase in autoreactivity. The parallel between metabolic outcome of human β-cell allografts and cellular auto- and alloreactivity in peripheral blood suggests a causal relationship. The present study therefore demonstrates that T-cell reactivities in peripheral blood can be used to monitor immune mechanisms, which influence survival of β-cell allografts in diabetic patients.

UR - http://www.scopus.com/inward/record.url?scp=0032971910&partnerID=8YFLogxK

U2 - 10.2337/diabetes.48.3.484

DO - 10.2337/diabetes.48.3.484

M3 - Article

C2 - 10078547

AN - SCOPUS:0032971910

VL - 48

SP - 484

EP - 490

JO - Diabetes

JF - Diabetes

SN - 1939-327X

IS - 3

ER -