Automated microfluidic chromatin immunoprecipitation from 2,000 cells

Research output: Contribution to journalArticle

Abstract

Chromatin immunoprecipitation (ChIP) is a powerful assay used to probe DNA-protein interactions. Traditional methods of implementing this assay are lengthy, Cumbersome and require a large number of cells, making it difficult to study rare cell types Such its certain cancer and stem cells. We have designed a microfluidic device to perform sensitive ChIP analysis on low cell numbers in a rapid, automated fashion while preserving the specificity of the assay. Comparing ChIP results for two modified histone protein targets, we showed our automated microfluidic ChIP (AutoChIP) from 2,000 cells to be comparable to that of conventional ChIP methods using 50,000-500,000 cells. This technology may provide a solution to the need for a high sensitivity, rapid, and automated ChIP assay. and in doing so facilitate the use of ChIP for many interesting and valuable applications.

Details

Authors
  • Angela R. Wu
  • Joseph B. Hiatt
  • Rung Lu
  • Joanne Attema
  • Neethan A. Lobo
  • Irving L. Weissman
  • Michael F. Clarke
  • Stephen R. Quake
Organisations
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Immunology in the medical area
Original languageEnglish
Pages (from-to)1365-1370
JournalLab on a Chip
Volume9
Issue number10
Publication statusPublished - 2009
Publication categoryResearch
Peer-reviewedYes