Bacterial glycosidases for the production of universal red blood cells

Research output: Contribution to journalArticle

Abstract

Enzymatic removal of blood group ABO antigens to develop universal red blood cells ( RBCs) was a pioneering vision originally proposed more than 25 years ago. Although the feasibility of this approach was demonstrated in clinical trials for group B RBCs, a major obstacle in translating this technology to clinical practice has been the lack of efficient glycosidase enzymes. Here we report two bacterial glycosidase gene families that provide enzymes capable of efficient removal of A and B antigens at neutral pH with low consumption of recombinant enzymes. The crystal structure of a member of the alpha-N-acetylgalactosaminidase family reveals an unusual catalytic mechanism involving NAD(+). The enzymatic conversion processes we describe hold promise for achieving the goal of producing universal RBCs, which would improve the blood supply while enhancing the safety of clinical transfusions.

Details

Authors
  • Qiyong P. Liu
  • Gerlind Sulzenbacher
  • Huaiping Yuan
  • Eric P. Bennett
  • Greg Pietz
  • Kristen Saunders
  • Jean Spence
  • Edward Nudelman
  • Steven B. Levery
  • Thayer White
  • John M. Neveu
  • William S. Lane
  • Yves Bourne
  • Martin L Olsson
  • Bernard Henrissat
  • Henrik Clausen
Organisations
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Hematology
Original languageEnglish
Pages (from-to)454-464
JournalNature Biotechnology
Volume25
Issue number4
Publication statusPublished - 2007
Publication categoryResearch
Peer-reviewedYes