Binding of dietary Cobalt to sarcoplasmic reticulum proteins

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Binding of dietary Cobalt to sarcoplasmic reticulum proteins. / Persson, B.; Carlenor, E.; Clyne, N.; Hultman, E.; Lins, L. E.; Pehrsson, S. K.; Rydström, J.

In: Scandinavian Journal of Clinical & Laboratory Investigation, Vol. 52, No. 2, 1992, p. 137-140.

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Persson, B. ; Carlenor, E. ; Clyne, N. ; Hultman, E. ; Lins, L. E. ; Pehrsson, S. K. ; Rydström, J. / Binding of dietary Cobalt to sarcoplasmic reticulum proteins. In: Scandinavian Journal of Clinical & Laboratory Investigation. 1992 ; Vol. 52, No. 2. pp. 137-140.

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TY - JOUR

T1 - Binding of dietary Cobalt to sarcoplasmic reticulum proteins

AU - Persson, B.

AU - Carlenor, E.

AU - Clyne, N.

AU - Hultman, E.

AU - Lins, L. E.

AU - Pehrsson, S. K.

AU - Rydström, J.

PY - 1992

Y1 - 1992

N2 - It has previously been shown that cobalt accumulates in the myocardium of rats, mainly the sarcoplasmic reticulum (SR) and the mitochondrial inner membrane. In order to investigate the mode of accumulation of cobalt in the SR, rats were given a dietary cobalt supplementation of 40 mg of CoSO4 × 7 H2O kg-1 body wt, after which the rats were sacrificed and the sarcoplasmic reticulum was isolated. The SR proteins were subjected to analysis by polyacrylamide gel electrophoresis followed by protein staining and determination of the content of cobalt in each protein band. The major cobalt-binding protein was found to have a molecular weight of about 100 000; a 200 000 molecular weight protein was also found to bind cobalt, although less extensively. These results suggest that cobalt is bound to the monomeric and dimeric forms of Ca2+-ATPase in the SR of the myocardium.

AB - It has previously been shown that cobalt accumulates in the myocardium of rats, mainly the sarcoplasmic reticulum (SR) and the mitochondrial inner membrane. In order to investigate the mode of accumulation of cobalt in the SR, rats were given a dietary cobalt supplementation of 40 mg of CoSO4 × 7 H2O kg-1 body wt, after which the rats were sacrificed and the sarcoplasmic reticulum was isolated. The SR proteins were subjected to analysis by polyacrylamide gel electrophoresis followed by protein staining and determination of the content of cobalt in each protein band. The major cobalt-binding protein was found to have a molecular weight of about 100 000; a 200 000 molecular weight protein was also found to bind cobalt, although less extensively. These results suggest that cobalt is bound to the monomeric and dimeric forms of Ca2+-ATPase in the SR of the myocardium.

KW - Congestive heart failure

KW - Metal ion binding

KW - Rat myocardium

UR - http://www.scopus.com/inward/record.url?scp=0026560892&partnerID=8YFLogxK

U2 - 10.3109/00365519209088777

DO - 10.3109/00365519209088777

M3 - Article

VL - 52

SP - 137

EP - 140

JO - Scandinavian Journal of Clinical & Laboratory Investigation

JF - Scandinavian Journal of Clinical & Laboratory Investigation

SN - 1502-7686

IS - 2

ER -