Bioactive compounds isolated from submerged fermentations of the Chilean fungus Stereum rameale

Research output: Contribution to journalArticle

Abstract

Liquid fermentations of the fungus Stereum rameale (No 2511) yielded extracts with antibacterial -activity. The antibacterial activity reached its peak after 216 h of stirring. Bioassay-guided fractionation methods were employed for the isolation of the bioactive metabolites. Three known compounds were identified: MS-3 (1), vibralactone (2) and vibralactone B (3). The three compounds showed antibacterial activity as a function of their concentration. Minimal bactericidal concentrations (MBC) of compound 1 against Gram-positive bacteria were as follows: Bacillus cereus (50 mu g/mL), Bacillus subtilis (10 mu g/mL) and Staphylococcus aureus (100 mu g/mL). Compounds 2 and 3 were active only against Gram-negative bacteria. The MBC of compound 2 against Escherichia coli was 200 mu g/mL. Compound 3 inhibited significantly the growth of E. coli and Pseudomonas aeruginosa, with MBC values of 50 and 100 mu g/mL, respectively.

Details

Authors
  • Pedro Aqueveque
  • Carlos Leonardo Cespedes
  • Jose Becerra
  • Marcelo Davila
  • Olov Sterner
Organisations
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Biochemistry and Molecular Biology

Keywords

  • antibacterial activity, MIC, MS-3, Stereum rameale, vibralactone
Original languageEnglish
Pages (from-to)97-102
JournalZeitschrift fuer Naturforschung. Section C: A Journal of Biosciences
Volume70
Issue number3-4
Publication statusPublished - 2015
Publication categoryResearch
Peer-reviewedYes