Biocompatible scaffolds based on natural polymers for regenerative medicine

Research output: Contribution to journalArticle

Abstract

The chitosan and gelatine are commonly used biopolymers for the tissue engineering applications. In the previous methods for the cryogels synthesis, multistep preparation methods using toxic cross-linking agents such as glutaraldehyde are reported. Here, we present a two-step preparation method of gelatin macroporous cryogels and one-step preparation method of chitosan or gelatin cryogels. The physico-chemical properties of obtained scaffolds were characterized using FTIR, zeta potential, SEM and laser confocal microscopy. Non-toxic and biodegradable cross-linking agents such as oxidized dextran and 1,1,3,3-tetramethoxypropane are utilized. The one-step chitosan cryogels had degradation degree ~2 times higher compared to the cryogels prepared with a two-step method i.e. reduced by borohydride. Scaffolds cross-linked by glutaraldehyde had about 40% viability, whereas nine various compositions of cryogels showed significantly higher viability (~80%) of fibroblast cells in vitro. The cryogels were obtained without using the harmful compounds and therefore can be used straightforward as biocompatible and biodegradable scaffolds for the cell culturing purposes and other biomedical applications.

Details

Authors
  • Dana Akilbekova
  • Madina Shaimerdenova
  • Salimgerey Adilov
  • Dmitriy Berillo
Organisations
External organisations
  • Nazarbayev University
  • University of Brighton
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Medicinal Chemistry

Keywords

  • 1,1,3,3-tetramethoxypropane, Chitosan, Cryogels, Natural polymers
Original languageEnglish
Pages (from-to)324-333
Number of pages10
JournalInternational Journal of Biological Macromolecules
Volume114
Publication statusPublished - 2018 Jul 15
Publication categoryResearch
Peer-reviewedYes