Biohydrogen production from wheat straw hydrolysate using Caldicellulosiruptor saccharolyticus followed by biogas production in a two-step uncoupled process

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Abstract

A two-step, un-coupled process producing hydrogen (H2) from wheat straw using Caldicellulosiruptor
saccharolyticus in a ‘Continuously stirred tank reactor’ (CSTR) followed by
anaerobic digestion of its effluent to produce methane (CH4) was investigated. C. saccharolyticus
was able to convert wheat straw hydrolysate to hydrogen at maximum production
rate of approximately 5.2 L H2/L/Day. The organic compounds in the effluent collected from
the CSTR were successfully converted to CH4 through anaerobic digestion performed in an
‘Up-flow anaerobic sludge bioreactor’ (UASB) reactor at a maximum production rate of 2.6 L
CH4/L/day. The maximum energy output of the process (10.9 kJ/g of straw) was about 57%
of the total energy, and 67% of the energy contributed by the sugar fraction, contained in
the wheat straw. Sparging the hydrogenogenic CSTR with the flue gas of the UASB reactor
((60% v/v) CH4 and (40% v/v) CO2) decreased the H2 production rate by 44%, which was due
to the significant presence of CO2. The presence of CH4 alone, like N2, was indifferent to
growth and H2 production by C. saccharolyticus. Hence, sparging with upgraded CH4 would
guarantee successful hydrogen production from lignocellulosic biomass prior to anaerobic
digestion and thus, reasonably high conversion efficiency can be achieved.

Details

Authors
  • Sudhanshu Pawar
  • Valentine Nkemka
  • Ahmad Zeidan
  • Marika Murto
  • Ed van Niel
Organisations
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Industrial Biotechnology

Keywords

  • Caldicellulosiruptor saccharolyticus Hydrogen CSTR Methane UASB Lignocellulosic biomass
Original languageEnglish
Pages (from-to)9121-9130
JournalInternational Journal of Hydrogen Energy
Volume38
Issue number22
Publication statusPublished - 2013
Publication categoryResearch
Peer-reviewedYes

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