Bioprospecting Microbial Diversity for Lignin Valorization: Dry and Wet Screening Methods

Research output: Contribution to journalReview article


Lignin is an abundant cell wall component, and it has been used mainly for generating steam and electricity. Nevertheless, lignin valorization, i.e. the conversion of lignin into high value-added fuels, chemicals, or materials, is crucial for the full implementation of cost-effective lignocellulosic biorefineries. From this perspective, rapid screening methods are crucial for time- and resource-efficient development of novel microbial strains and enzymes with applications in the lignin biorefinery. The present review gives an overview of recent developments and applications of a vast arsenal of activity and sequence-based methodologies for uncovering novel microbial strains with ligninolytic potential, novel enzymes for lignin depolymerization and for unraveling the main metabolic routes during growth on lignin. Finally, perspectives on the use of each of the presented methods and their respective advantages and disadvantages are discussed.


  • Carolyne Caetano Gonçalves
  • Thiago Bruce
  • Caio de Oliveira Gorgulho Silva
  • Edivaldo Ximenes Ferreira Fillho
  • Eliane Ferreira Noronha
  • Magnus Carlquist
  • Nádia Skorupa Parachin
External organisations
  • University of Brasilia
  • Universidade Católica de Brasília
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Biocatalysis and Enzyme Technology


  • activity-based screening, bioprocess, biosensors, high-throughput screening, lignin-degrading enzymes
Original languageEnglish
Article number1081
JournalFrontiers in Microbiology
Publication statusPublished - 2020 Jun 9
Publication categoryResearch