Blinking statistics of a molecular beacon triggered by end-denaturation of DNA
Research output: Contribution to journal › Article
We use a master equation approach based on the Poland–Scheraga free energy for DNA denaturation to investigate the (un)zipping dynamics of a denaturation wedge in a stretch of DNA that is clamped at one end. In particular, we quantify the blinking dynamics of a fluorophore–quencher pair mounted within the denaturation wedge. We also study the behavioural changes in the presence of proteins that selectively bind to single-stranded DNA. We show that such a set-up could be well suited as an easy-to-implement nanodevice for sensing environmental conditions in small volumes.
|Research areas and keywords||
Subject classification (UKÄ) – MANDATORY
|Journal||Journal of Physics: Condensed Matter|
|Publication status||Published - 2005 Dec 14|