Block in insulin release from column-perifused pancreatic β-cells induced by islet cell surface antibodies and complement

Research output: Contribution to journalArticle

Abstract

Dispersed rat pancreatic islet cells were mixed into a short column of Bio-Gel P-2 polyacrylamide beads and perifused with an antiserum containing islet cell surface antibodies. The release of radioactive chromium from prelabeled cells, as a measure of cell membrane permeability, was not affected by cell surface antibodies alone, but increased dramatically in the presence of complement. While there was an eightfold increase in glucose-stimulated insulin release from β-cells exposed to control serum and complement, insulin release was completely blocked from β-cells exposed to islet-cell-specific antibodies and complement. These findings suggest that islet cell surface antibodies can mediate complement-dependent cytotoxicity.

Details

Authors
External organisations
  • University of Chicago
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Cell and Molecular Biology
Original languageEnglish
Pages (from-to)231-234
Number of pages4
JournalDiabetes
Volume30
Issue number3
Publication statusPublished - 1981 Jan 1
Publication categoryResearch
Peer-reviewedYes
Externally publishedYes