Calcium-dependent self-association of the C-type lectin domain of versican

Research output: Contribution to journalArticle

Abstract

Versican is a large (1-2 x 10(6) Da) chondroitin-sulfate proteoglycan that can form large aggregates by means of interaction with hyaluronan and also binds to a series of other extracellular matrix proteins, chemokines and cell-surface molecules. Versican is a multifunctional molecule with roles in cell adhesion, matrix assembly, cell migration and proliferation. Characterization of the binding interactions mediated by the various domains of versican is a first step towards understanding the functions of versican and interacting molecules in the extracellular matrix. In this study we investigated a recombinant construct corresponding to the C-type lectin domain of versican and demonstrated a calcium-dependent self-association of this region by blot overlay and plasmon surface resonance assays. Electron microscopy provided further evidence of the relevance of the binding reaction by demonstrating a mixture of monomers, dimers and complex aggregates of recombinant versican C-type lectin domain. This binding reaction could contribute to the ability of versican to organize formation of the proteoglycan extracellular matrix by inducing binding of individual versican molecules or by modulating binding reactions to other matrix components.

Details

Authors
  • A Ney
  • P Booms
  • G Epple
  • Matthias Mörgelin
  • G Guo
  • G Kettelgerdes
  • R Gessner
  • PN Robinson
Organisations
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Infectious Medicine

Keywords

  • connective tissue, extracellular matrix, surface plasmon resonance, versican, C-type lectin domain, electron microscopy
Original languageEnglish
Pages (from-to)23-29
JournalInternational Journal of Biochemistry & Cell Biology
Volume38
Issue number1
Publication statusPublished - 2006
Publication categoryResearch
Peer-reviewedYes