Cambios geneticos en cepas de poliovirus tipo 2 aisladas de pacientes con poliomielitis paralitica asociada a la vacuna

Research output: Contribution to journalArticle

Abstract

INTRODUCTION: Poliomyelitis is currently a rare disease in developed countries, where only vaccinal strains seem to be in circulation, which replace wild poliovirus. Nevertheless, it is still a serious disease for children in underdeveloped countries of Asia and Africa.

PATIENTS AND METHODS: We analysed nine strains of poliovirus type 2 isolated from the faecal matter of patients with vaccine associated paralytic poliomyelitis (VAPP), from the beginning of anti polio vaccination campaigns in our country. These strains were submitted to sequencing of a fragment of 114 base pairs from the 5 NTR (non traductional region), where one of the main determinants of attenuation/reversion to the neurovirulence of poliovirus lies in the position of nucleotide 481.

RESULTS: In this position it was observed how guanine had been replaced by adenine in all the strains that were sequenced, so that it coincided with the homologous sequence of the wild strain, as well as with that of strains obtained from healthy children immunised with the live vaccine. This presupposes that other changes must occur or that other factors must be involved for VAPP to occur or not, and we therefore suggest the sequencing of other regions of the genome in search of other possible differential changes in nucleotides.

Details

Authors
External organisations
  • Pedro Kouri Tropical Medicine Institute (IPK)
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Microbiology in the medical area

Keywords

  • Child, Child, Preschool, Humans, Point Mutation/genetics, Poliomyelitis, Bulbar/prevention & control, Poliovirus/genetics, Poliovirus Vaccine, Oral/administration & dosage, Reverse Transcriptase Polymerase Chain Reaction, Vaccines, Attenuated/administration & dosage
Translated title of the contributionGenetic changes in strains of poliovirus type 2 isolated from patients with vaccine-associated paralytic poliomyelitis
Original languageSpanish
Pages (from-to)812-6
Number of pages5
JournalRevista de Neurologia
Volume36
Issue number9
Publication statusPublished - 2003 Apr 30
Publication categoryResearch
Peer-reviewedYes
Externally publishedYes