Capacitive immunosensor for the detection of host cell proteins.

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Abstract

A new analysis for monitoring host cell proteins in preparations of transgenically produced protein pharmaceuticals is described. A capacitive biosensor with a very high sensitivity is used to monitor trace amounts of host cell proteins. The sensor consists of a gold electrode, the surface of which is well insulated and on which a preparation of a population of polyclonal antibodies raised against the complete protein set-up of the host cell. Host cell proteins are present at very low concentrations during the production of a transgenic protein. The system studied here is a mode system with an enzyme expressed in Escherichia coli (E. coli). Due to the high sensitivity, it may even be possible to dilute the samples to be analyzed, thereby reducing negative influence from non-specific binding to the sensor surface.

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  • Industrial Biotechnology
Original languageEnglish
Pages (from-to)207-213
JournalJournal of Biotechnology
Volume157
Publication statusPublished - 2012
Publication categoryResearch
Peer-reviewedYes