Capacitive Saccharide Sensor Based on Immobilized Phenylboronic Acid with Diol Specificity

Research output: Contribution to journalArticle

Abstract

A capacitive sensor for saccharide detection is described in this study. The detection is based on selective interaction between diols and aminophenylboronic acid (APBA) immobilized on a gold electrode. Glucose, fructose, and dextran (MW: 40 kDa) were tested with the system over wide concentration ranges (1.0 x 10−8 M - 1.0 x 10−3 M for glucose, 1.0 x 10−8 M - 1.0 x 10−2 M for fructose and 1.0 x 10−10 M - 1.0 x 10−5 M for dextran). The limits of detection (LODs) were 0.8 nM for glucose, 0.6 nM for fructose, and 13 pM for dextran. These data were comparable to the others reported previously. In order to demonstrate glycoprotein detection with the same sensor, human immunoglobulin G (IgG) as well as horseradish peroxidase were used as model analytes. The sensor responded to IgG in the concentration range of 1.0 x 10−13 M - 1.0 x 10−7 M with a LOD value of 16 fM. The performance of the assay of peroxidase was compared to a spectrophotometric assay by determining the enzymatic activity of a captured analyte. The results showed that the method might be useful for label-free, fast, and sensitive detection of saccharides as well as glycoproteins over a wide concentration range.

Details

Authors
Organisations
External organisations
  • CapSenze Biosystems AB
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Biochemistry and Molecular Biology

Keywords

  • Aminophenylboronic acid, Capacitive sensor, Glycoprotein detection, Saccharide detection
Original languageEnglish
JournalApplied Biochemistry and Biotechnology
Publication statusE-pub ahead of print - 2018 Oct 28
Publication categoryResearch
Peer-reviewedYes