Capacitive Sensor to Monitor Enzyme Activity by Following Degradation of Macromolecules in Real Time

Research output: Contribution to journalArticle

Abstract

A capacitive sensor was developed to analyze the presence and enzymatic activity of a model protease from standard solutions by following the degradation of the substrate in real time. The enzyme was chosen based on its specific digestion of the hinge region of immunoglobulin G (IgG). Real-time enzyme activity was monitored by measuring the change in capacitance (∆C) based on the release of IgG fragments after enzymatic digestion by the enzyme. The results indicated that the developed capacitive system might be used successfully for label-free and real-time monitoring of enzymatic activity of different enzymes in a sensitive, rapid, and inexpensive manner in biotechnological, environmental, and clinical applications.

Details

Authors
Organisations
External organisations
  • CapSenze Biosystems AB
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Biomedical Laboratory Science/Technology
  • Medical Laboratory and Measurements Technologies

Keywords

  • Capacitive sensor, Degradation, Enzymatic activity, Protease
Original languageEnglish
JournalApplied Biochemistry and Biotechnology
Publication statusE-pub ahead of print - 2019
Publication categoryResearch
Peer-reviewedYes