Carbon ceramic electrodes modified with laccase from Trametes hirsuta: Fabrication, characterization and their use for phenolic compounds detection

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Carbon ceramic electrodes modified with laccase from Trametes hirsuta: Fabrication, characterization and their use for phenolic compounds detection. / Haghighi, Behzad; Rahmati-Panah, Amir; Shleev, Sergey; Gorton, Lo.

In: Electroanalysis, Vol. 19, No. 9, 2007, p. 907-917.

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TY - JOUR

T1 - Carbon ceramic electrodes modified with laccase from Trametes hirsuta: Fabrication, characterization and their use for phenolic compounds detection

AU - Haghighi, Behzad

AU - Rahmati-Panah, Amir

AU - Shleev, Sergey

AU - Gorton, Lo

N1 - The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004)

PY - 2007

Y1 - 2007

N2 - Fungal laccase (Lc) from the basidiomycete Trametes hirsuta was immobilized on top of a carbon ceramic electrode using physical absorption. Direct, unmediated heterogeneous electron transfer between Lc and the carbon ceramic electrode (CCE) under aerobic conditions was shown. The bioelectrocatalytic reduction of oxygen on Lc-CCE started at about 430 mV vs. Ag vertical bar AgCl vertical bar KClsat at pH 3.5 and moved with about 57 mV in the cathodic region per pH unit. The Lc-modified CCE was then used as a biosensing detection element in a single line flow injection system for the amperometric determination of a variety of phenolic substrates of the enzyme. The experimental conditions were studied and optimized for catechol serving as a model compound. Statistical aspects were applied and the sensor characteristics and Michaelis-Menten constants of the investigated phenolic compounds were calculated and compared with those obtained for solid graphite electrodes modified with Trametes hirsuta laccase. The results showed that the CCE based biosensor in comparison with the solid graphite based biosensor offers a lower detection limit, a wider linear dynamic range, and excellent operational stability with no sensor passivation, indicating that the sol - gel lattice improves the electrochemical behavior of the biosensor.

AB - Fungal laccase (Lc) from the basidiomycete Trametes hirsuta was immobilized on top of a carbon ceramic electrode using physical absorption. Direct, unmediated heterogeneous electron transfer between Lc and the carbon ceramic electrode (CCE) under aerobic conditions was shown. The bioelectrocatalytic reduction of oxygen on Lc-CCE started at about 430 mV vs. Ag vertical bar AgCl vertical bar KClsat at pH 3.5 and moved with about 57 mV in the cathodic region per pH unit. The Lc-modified CCE was then used as a biosensing detection element in a single line flow injection system for the amperometric determination of a variety of phenolic substrates of the enzyme. The experimental conditions were studied and optimized for catechol serving as a model compound. Statistical aspects were applied and the sensor characteristics and Michaelis-Menten constants of the investigated phenolic compounds were calculated and compared with those obtained for solid graphite electrodes modified with Trametes hirsuta laccase. The results showed that the CCE based biosensor in comparison with the solid graphite based biosensor offers a lower detection limit, a wider linear dynamic range, and excellent operational stability with no sensor passivation, indicating that the sol - gel lattice improves the electrochemical behavior of the biosensor.

KW - electrochemical

KW - carbon ceramic electrode

KW - phenol

KW - biosensor

KW - laccase

KW - behavior

U2 - 10.1002/elan.200603839

DO - 10.1002/elan.200603839

M3 - Article

VL - 19

SP - 907

EP - 917

JO - Electroanalysis

JF - Electroanalysis

SN - 1040-0397

IS - 9

ER -